Data/hora: 01/06/2024 15:09:43

Biblioteca(s): | Epagri-Sede.
Tipo da produção científica: | Resumo em Anais de Congresso
Data corrente: | 18/11/2022
Data da última atualização: | 18/11/2022
Autoria: | ATHAYDE, A. E.; ARAUJO, C. E. S.; SANDJO, L. P.; BIAVATTI, M. W.
Título: | Metabolomic comparison between wild and cultivated Porophyllum ruderale Jacq. Cass (Asteraceae) using UPLCESI-QTof-MS technique.
Ano de publicação: | 2022
Fonte/Imprenta: | In: LATIN AMERICAN METABOLIC PROFILING SOCIETY MEETING, 4., 2022, Cartagena, Colombia. Abstracts... Cartagena, Colombia: LAMPS, 2022.
Idioma: | Inglês

Conteúdo:

Porophyllum ruderale Jacq. Cass (Asteraceae) is a non-convention edible plant found in south of Brazil with interesting and complex chemical profile. Objectives - Thus, the
present study phytochemically explore P. ruderale and compare individuals of wild and cultivated plant samples. Results: UPLC-ESI-QToF-MS technic afforded putatively
identification of 36 substances including mainly phenolic acids, fatty acids and chlorophyll derivatives. Of that, 21 substances were tentatively identified as diterpene
aminoglycoside wedeloside derivatives, described for the first time in P. ruderale. Designed for that, PCA (principal component analysis) and cluster analysis were used to
distinguish different metabolites among wild and cultivated P. ruderale samples, that were separated into well-defined groups. The principal components (PCs) of all detected
signals in positive mode (n=3846) where computed in order to reduce dimensionality and eliminate redundancy. The algorithm centered the data and used the singular value
decomposition (SVD) to compute the PCs. Working with the firsts 23 PCs, that explains more than 99% of the dataset variance, was built an hierarchical binary cluster tree. 
A kmeans clustering algorithm was also used to check the clustering partitioning coherence.The optimum number of clusters for the k-means method was determined as eight
clusters. Conclusions - Finally, PC3 cumulative variance could explain more than 81% of the total data variance and the ions m/z 918, 920 and 962 influenced the PC3 cumulative
percent on 99%, 99%, 97% and 97%, respectively. These ions correspond to new skeletons of diterpene aminoglycosides, supported by tandem mass (MS2) fragmentation
profile. Future perspectives: Unequivocally elucidate the identified diterpene aminoglycoside wedeloside derivatives through chromatographic isolation, and fully
characterize those using spectrometric technics, especially NMR.

Thesagro: | diterpene aminoglycosides; K-means analysis; Porophyllum ruderale; Principal Component Analysis; UPLC-ESI-QToF-MS.
Categoria do assunto: | X Pesquisa, Tecnologia e Engenharia
URL: | http://intranetdoc.epagri.sc.gov.br/producao_tecnico_cientifica/DOC_55676.pdf
Registro original: | Epagri-Sede (Epagri-Sede)
Exemplares: | Epagri-Sede - (107741-1) - UPC - PL - DD -  -  -  - DIS